AI image recognition algorithm quickly identifies high-quality single cells
Biodispensing Cartridge
The single cell dispensing cartridge, which is the core component of theplatform,is composed of intelligent microfluidic chip that integratesCMOS and MEMS technologies to achieve integrated, miniaturized andhigh-throughput signal sensing, signal processing and liquid handling.
>>Highly redundant and independently controllable nozzles avoidspotential issues with individual channel clogging
>>Completely free-of-tubing design means low shear and no harmto cell viability
>>Disposable cartridges removes risk of cross contamination
Single cells are gently dispensedinto a 96-well plate in 2 minutes orinto a 384-well plate in 8 minutes.
Al-powered image recognition discriminates good quality cellsfrom poor quality ones,ensuring ahigher clonal recovery rate.
Capture nozzle images for everydispensing run to assure thatonly single cell was dispensedinto the plate.
Eliminate the risk of cross contamination during dispensingwith single-use cartridges.
Plug-and-play cartridge with noneed for manual adjustment maintenance-free operation, enabledby completely tubing less design.
A single cartridge containingover 100 parallel working nozzlesavoids experiment failure due toclogging lssues.
| Single cell rate | ≥98%,based on Al-powered single cell identification |
| Single-cell recognition algorithm | Cell diameter, cell roundness, and other parameters; flexible matching with convolutional neural networks (CNN) to enhance single-cell recognition and screening efficiency for user cell samples |
| Compatible sample types | Cell lines (CHO, HEK, various tumor cell lines, etc.), iPSCs, primary cell samples, cell nuclei, protoplasts, etc |
| Monoclonality assurance | >99.5% |
| Imaging capablllty | Brightfield, numerial aperture =0.3 |
| Single cell dispensingprocessing times | ~2min/96-well plate:~8min/384-well plate |
| Reference Well Setup | Allow users to set a reference well, which involves adding multiple cells to a specific well to facilitate focusing references in subsequent well plate imaging |
| Number of nozzles | 640, separate control |
| Plate coating buffer | Supports serum-containing culture medium, PBS, water, ethanol, etc |
| Nozzle size | 30μm |
| Droplet volume | 30pL |
| Collection devices | 6/12/24/48/96/384 well-plates, 8-strip tubes.microslides,customized matrix |
| Dimensions | W:650mm x D: 410mm x H: 330mm |
| Operating temperature | 15'℃ to 30'℃ |
| Relative humidity | 20% to 80% |
